Name of instrument, m RealTime System composed of the msp and mrt modules. Country where designed/Manufactured/Reagents. The Abbott m System comprises the msp and mrz instruments, which are operated with separate System Control Center (SCC). J Clin Microbiol. Jun;47(6) doi: /JCM Epub Apr Performance of the Abbott real-time PCR assay using msp and .
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Results of this study are unable to evaluate the potential differences between these two assays in this regard. The observed differences could potentially abboyt due to a number of intrinsic differences between these two assays, e.
Please leave a message, we will get back you shortly. Four different sample extraction volumes 0. The Abbott method positively identified samples out of with the detectability of Guidelines Upcoming Special Issues.
Short Communication Open M20000sp. The difference between these two methods was Log10 0. Thus some of the discordant samples could potentially be due to the HIV-1 subtype differences. The number of samples within this range was Detection by real-time technology used two probes of different lengths: Future work includes increase of the testing population size, determination of the HIV-1 subtypes and DNA sequence analysis of those discrepant samples.
The vertical axis represenst the VL differences the Abbott method minus the Roche method and the horizontal axis represenst the means of VL. Why request a quote through SelectScience? Of the ND Roche m2000s, 15 Fast Your inquiry will be delivered straight to the manufacturer. This automation enables laboratorians to interface where their skills best contribu In addition, different precision of the methods could also potentially explain some of the discordant results.
The extracted NA was mixed with the amplification reagents before amplification with the mrt Abbott, a ; Abbott, All blood samples were collected in EDTA tubes. Bland-Altman analysis of the quantitative results.
m RealTime System from Abbott | SelectScience
Our data showed that these two methods are essentially indistinguishable with a correlation coefficient of 0. Be the first to review this product. These two assays share three common features 1 Additional reduction in the lower limits of detection LOD of HIV-1 RNA from the earlier version; 2 New primers and probes designs for recognition of the different viral subtypes and circulating recombinant forms CRFs ; and 3 Reduction in hand-on time by configuring the test n2000sp to full automation.
The Version 2 of the m open mode 0. The plot to the left represents the distribution of the VL from the Abbott method assay and the plot to the right represents the distribution of VL from the Roche assay.
Comparison of HIV-1 Viral Load between Abbott m2000 and Roche COBAS TaqMan Methods
The Abbott mrt performed the automated amplification and detection process. Detection of the resulting fluorescence collectively provides an immediate real-time quantification of the PCR process.
Home Publications Conferences Register Contact. This automation enables laboratorians to interface where their skills best contribute to results while minimizing errors and contamination.
All VL values were first transformed into the log10 format. However, comparison of the differences of VL measurements between the two methods by using the Bland Altman plot showed rough symmetric distribution of the differences and the log differences in Ann Jose ankara escort. Whether through new system software releases, innovative assay design or a constantly expanding mSystem menu, we are continuously evolving. October 18, Citation: Out of paired plasma tested, Other assay performance characteristics such as analytical specificity, linearity, reproducibility, and precision were not evaluated.
In this configuration, signal is not created by probe hydrolysis but by separation of the probe from quencher oligonucleotide.
Abbott M2000sp Liquid Handler W/ M2000rt PCR
The best fi t for the regression analysis is indicated by the solid line. Digestion of the probe DNA separates the reporter dye from the quencher dye, making the reporter dye signal detectable. Agri and Aquaculture Journals Dr. The Abbott method uses real-time platforms with fluorescence detection systems Abbott, b ; Scott et al. It is unclear at the moment the source of the discrepancies. For examples, avbott Abbott method has been shown to have better detectability of non-B subtypes Geelen et al.
This method has now become a standard method for monitoring HIV-infected patients on antiretroviral therapy in the United States. Table 1 summarizes the assay characteristics of these two assays.