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Part : HD74163P,HD74170R-P,HD74175P
Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Protocol To access the protocol, please provide your email address: X Need an answer now? Prices are subject to change without notice.
Soldering Tips Helpful Link: Contact us Chat with our specialists Contact your local tebu-bio office Meet us there Business developpment Follow us. Tris Base Chemzymes Ultra Pure. Depending on the species used to produce the primary antibody: Sonicate for 10—15 sec using a probe sonicator to shear DNA. Quantify total protein content in the lysate using Total Protein Determination Kit Maccording to the manufacturer’s instructions. Wash nitrocellulose membrane two times with dH2O and then stain the nitrocellulose datashset with Ponceau S Staining Solution with gentle agitation.
(PDF) Datasheet PDF Download – 4 x 4 Register File
For details on these services, please click the appropriate link from the menu on the left. San Diego Blood Bank. At this point, the membrane can be evaluated to determine if equivalent amounts of protein were loaded in each lane.
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datasheet pdf download
Adjust pH to 6. A 10mL pipette should be used to gently roll out bubbles after the last blotting paper is laid on the stack. Fill the chamber up until you reach a point that will be 1cm below the bottom of the gel comb when it is added in the next step. Remove the Ponceau S Staining Solution and wash the membrane with dH2O at least three times and visualize cellular proteins.
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Anti Apolipoprotein CIII (Apo CIII)
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You may wish to photograph or scan the stained membrane or to cut the membrane horizontally so that you can use one primary antibody on the top of the membrane and another on the bottom of the membrane.
Open Positions To see a list of open positions, click here. Total Datashheet Determination kit. Spring Semester, Monday — Friday: Secondary Antibody Dilution Buffer mL: