Specialized hydrocarbonoclastic bacteria (SHCB), which use limited carbon sources with a preference for petroleum hydrocarbons, were found. Petroleum microbiology is a branch of microbiology that deals with the study of microorganisms that can metabolize or alter crude or refined petroleum products. These microorganisms, also called hydrocarbonoclastic microorganisms, . ” Most Hydrocarbonoclastic Bacteria in the Total Environment are Diazotrophic, which. The marine hydrocarbonoclastic bacterium Alcanivorax borkumensis is able to degrade mixtures of n-alkanes as they occur in marine oil spills. However.

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The pure isolated bacteria strains were identified morphologically and on the basis of their biochemical reactions in the Department of Biological Sciences, Bactera.

Publish Your Research Bacteriz. The percentage loss of total peak areas in the GLC chromatograms based on the peak areas of the control samples similarly prepared, but using previously autoclaved bacteria was calculated as a quantitative measure of oil consumption. May 17, ; Published: Phytoremediation for oily desert soil.

Growth in terms of optical density at nm was measured at time zero and in 2-day intervals. An amount of 0.

Potential Hydrocarbonoclastic Bacteria

Inthe Exxon Valdez ran aground, spilling There are 74 active users right now. Improvement in the RFLP procedure for studying the diversity of nifH genes in communities of nitrogen fixers in soil. Indigenous hydrocarbon-utilizing bacterioflora in oil-polluted habitats in Kuwait, two decades after the greatest man-made oil spill. Oil consumption was also markedly effective in the NaNO 3 -containing medium with and without nitrogen gas in bacteeria head space.

Microbiology and Molecular Biology Reviews. The treatment methods used for remediation include physical, chemical and biological processes. Culture-independent approach DGGE showed high diversity in the microbial community in all the studied samples with a clear correlation with the hydrocarbon pollution rate.


Society of Petroleum Engineers. The occurrence, in all samples, of two gene bands instead of only one Fig.

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Therefore, we assumed that, in the course of early evolution, microorganisms that developed the ability hydroccarbonoclastic utilize nitrogen-poor substrates, such as oil, simultaneously developed the potential for nitrogen fixation in order to synthesize their nitrogenous cell materials including proteins and nucleic acids.

These criteria have been used to confirm diazotrophic potential rather than actual in situ nitrogen fixation during growth on hydrocarbon substrates as sources of carbon and energy. Materials and Methods Bacterial isolates One hundred different species of oil-utilizing bacteria that have been deposited in our private culture collection were used in this study. Table 3 Crude oil-consumption by bacterial species in the presence and absence of NaNO 3 in the medium and nitrogen gas in the culture top space.

Table 1 shows the names of the 15 species selected for further study, as well as information related to their 16S rDNA sequencing.

Bushnell-Haas Agar was used to culture the crude oil degrading bacteria. The potential of oil-utilizing consortia associated with legume root nodules for cleaning oily soils.

Hydrocarbono-clastic means degrading hydrocarbons or simply ‘eating’ or ‘breaking’ the hydrocarbon molecules. Neptunomonas naphthovorans degrade polyaromatic hydrocarbons PAH especially naphthalene.

One hundred-milliliter aliquots of the mineral medium 24 amended with 0. The cultures were sealed and incubated on an electric shaker at rpm for 2 weeks. However, redox potential, hydrocaarbonoclastic, light intensity and concentration of hydrocarbon substrate largely affect growth and activity of HCB. Crude oil consumption by individual bacterial species was measured quantitatively in batch cultures under the conditions specified above.

Important role of these polymeric byproducts in the conversion of hydrocarbons to bioavailable form is principally responsible for their hydrocarbonoclastic potential in the soil. From Wikipedia, the free encyclopedia. On the contrary, Thalassolituus oleivorans can grow on and degrade only aliphatic hydrocarbons.


Hydrocabonoclastic bacteria are referred to as bacteria hydrocarbonoclatic can biodegrade hydrocarbon. It constitutes the primary mechanism for the elimination of hydrocarbons from contaminated sites by natural existing populations of microorganisms.

Atlas RM, Bartha R. Abstract Eighty-two out of the hydrocarbonoclastic bacterial species that have been already isolated from oil-contaminated Kuwaiti sites, characterized by 16S rRNA nucleotide sequencing, and preserved in our private culture collection, grew successfully in a mineral medium free of any nitrogenous compounds with oil vapor as the sole carbon source.

When further supplied with sufficient limiting nutrients such as nitrogen baacteria phosphor, it grows and produces surfactant glucolipids to help reduce surface water tension and enhance hydrocarbon uptake.

Microbiological degradation of hydrocarbons in the environment. Nitrogen fertilizers have been shown to limit bioremediation bbacteria oily environments 57810112122 Soil bacteria mentioned above are also found in freshwater as they are carried by rainwater runoff, water drainage or via discharge of effluents.

Emulsifiers produced by microbes can break up oil into smaller droplets, effectively increasing the available surface area. COD reduction by bacterial isolates from Refinery effluent. Nutrient broth and effluent were added in a ratio of 4: The microbial utilization of such carbon-rich, but nitrogen-poor substrates necessitates the availability of nitrogenous compounds for the synthesis of cell hydorcarbonoclastic such as proteins and nucleic acids.

They synthesize extracellular abcteria like polyhydroxy alkanoates and wax esters when they are grown on sole carbon source like hexadecane under phosphorus and nitrogen limiting conditions. Hydrocarbonoclastic potential of HCB in freshwater is dependent on emulsification or surfactant activity of strains, pH, concentration of contaminant in that habitat.

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